Antitumor mamayewa da metastasis na Agkistrodon acutus venom bangaren ACTX-8 a kudancin Anhui Bincike kan aiki

Makasudi Don bincika sakamako da lokaci na bangaren Agkistrodon acutus venom ACTX-8 akan mannewa da mamaye kwayar cutar kansar mahaifa Hela.

Tsarin rufewa.Hanyoyin Fibronectin an rufe su a cikin farantin rijiyar 96.Kwayoyin Hela sun wuce ta 0, 2.5, 5, 10, 20, 40 g/ml na ACTX-8

Bayan jiyya, an gano mannewa tare da Fibronectin, kuma MTY ya gano adadin adadin kwayoyin halitta;Gano mamayewar tantanin halitta da ƙaura ta hanyar tantanin halitta ta TransweU

Canje-canje a cikin iyawa;Haɗin gasa tsakanin ACTX-8 da Fibronectin da membrane cell Hela an gano su ta hanyar cytometry mai gudana.Sakamako:

Bayan jiyya tare da ACTX-8, mannewar kwayoyin Hela zuwa Fibronectin ya ragu, kuma gwajin kwayar halitta ya nuna cewa kwayoyin Hela sun yi hijira.

Hakanan an rage karfin ƙaura da mamayewa.Sakamakon cytometry mai gudana ya nuna cewa ACTX-8 na iya yin gasa don ɗaure mai karɓar Fibronectin akan tantanin halitta.