Don lura da tasirin ƙwayar ƙwayar cuta ta I na Agkistrodon acutus venom (AAVC-1) akan ayyukan ƙaura na sel endothelial na jikin mutum (HUVECs), da kuma gano yiwuwar tsarin AAVC - Ⅰ hana angiogenesis.Hanyoyi: An haɓaka HUVECs a cikin vitro tare da AAVC - Ⅰ (0, 20, 40, 80 μ G/ml) an haɗa ƙwayoyin da aka yi da su don 24 h.An yi amfani da gwajin gwaji da gwajin gwaji na chemotactic don lura da tasirin AAVC - Ⅰ akan ayyukan ƙaura na endothelial;An yi amfani da RT-PCR da Western blot don gano sauye-sauye na mRNA da matakan furotin na P-selectin da abubuwan mannewa tsakanin salula (ICAM-1) kafin da bayan maganin miyagun ƙwayoyi.Sakamako: Idan aka kwatanta da HU VECs a cikin ƙungiyar al'ada, ƙarfin ƙaura na sel a cikin AAVC - Ⅰ ƙungiyoyi masu tattarawa sun ragu zuwa digiri daban-daban, kuma bayanin P-selectin da ICAM-1 mRNA ya ragu sosai.Kammalawa: AAVC - Ⅰ na iya hana ayyukan ƙaura na sel endothelial ta hanyar saukar da mRNA da matakan furotin na P-seletin da ICAM-1
Lokacin aikawa: Dec-07-2022