Tasirin Ciwon Tumor Sashi na I na Agkistrodon acutus Venom akan Ayyukan Hijira na Kwayoyin Endothelial na Farji.

Manufa: Don lura da tasirin anti-tumor bangaren I na Agkistrodon acutus venom (AAVC-1) akan ayyukan ƙaura na sel endothelial na jikin mutum (HUVECs), da kuma gano hanyar da za ta yiwu na AAVC - Ⅰ hanawa. angiogenesis.Hanyoyi: An haɓaka HUVECs a cikin vitro kuma an bi da su tare da AAVC - Ⅰ (0, 20, 40, 80 μ G / ml) an shigar da ƙwayoyin da aka kula da su don 24 h.An yi amfani da gwajin gwaji da gwajin gwaji na chemotactic don lura da tasirin AAVC - Ⅰ akan ayyukan ƙaura na endothelial;An yi amfani da RT-PCR da Western blot don gano sauye-sauye na mRNA da matakan furotin na P-selectin da abubuwan mannewa tsakanin salula (ICAM-1) kafin da bayan maganin miyagun ƙwayoyi.Sakamako: Idan aka kwatanta da HU VECs a cikin ƙungiyar al'ada, ƙarfin ƙaura na sel a cikin AAVC - Ⅰ ƙungiyoyi masu tattarawa sun ragu zuwa digiri daban-daban, kuma bayanin P-selectin da ICAM-1 mRNA ya ragu sosai.Kammalawa: AAVC - Ⅰ na iya hana ayyukan ƙaura na sel endothelial ta hanyar saukar da mRNA da matakan furotin na P-seletin da ICAM-1