Ƙaddamar da ƙananan ƙwayoyin thrombin kamar enzyme Agapurin daga Agkistrodon halys venom da jerin ragowar amino acid.

Manufa Don tantance ragowar amino acid guda 15 na N-terminal na Agacutin.Subunit guda biyu na kwayoyin halittar enzyme suna buƙatar a raba su don samun sarkar peptide subunit guda ɗaya kuma a jera su.An samo hanyoyin Agacutin da aka tsarkake sosai ta hanyar sinadarai.Samfurori guda ɗaya don ganowa da jeri an shirya su ta hanyar SDS-PAGE electrophoresis separation, subnit m tsiri dawo da da PVDF membrane electrotransfer fasahar.Ƙarshen ya ƙaddamar da ragowar amino acid 15 a N-terminal na subunit ta hanyar lalata Edman.Sakamako Jeri na babban subunit (16 kDa) shine DCSSGWSSYEEHQYY, kuma na ƙaramin sashi (15 kDa) shine DSSGWSSYEGHEYYV.Ƙarshe Sakamakon jeri ya nuna cewa Agacutin wani sabon macijin dafin thrombin ne kamar enzyme ta hanyar binciken bayanan NCBI.