Manufa: Don nazarin tasirin ƙwayar cuta na ƙwayar cuta I (AAVC-I) akan haɓakar haɓakawa da kuma apoptosis na ciwon huhu na mutum A549 Kwayoyin.Hanyoyi: An yi amfani da hanyar MTT don gano ƙimar hanawar 24h da 48h na ƙwayoyin A549 ta AAVC-I a wurare daban-daban.
HE tabo da Hoechst33258 mai kyalli da aka yi amfani da su don lura da apoptosis daga ilimin halittar jiki;An yi amfani da Immunohistochemistry don gano canje-canje a cikin maganganun furotin BAX.Sakamako: MTT ya nuna cewa AAVC-I na iya hana yaduwar kwayoyin A549 a cikin lokaci-da kuma dogara da kashi.Bayan jiyya na AAVCI don 24h, ƙanƙancewar tsakiya, hyperstaining nukiliya da jikin apoptotic sun kasance a bayyane a ƙarƙashin microscopy.Immunohistochemistry ya nuna cewa matsakaicin ƙimar gani na gani ya karu tare da karuwar ƙwayar ƙwayoyi, yana nuna cewa bayanin furotin BAX ya daidaita daidai.
Lokacin aikawa: Mayu-11-2023